Maybe the secret of Bravo.
The Alpha-Lactalbumin/Oleic Acid Complex and Its Cytotoxic Activity
It has been theorized that HAMLET could be naturally formed in the digestive system of
breast-fed children, due to low pH conditions of the stomach, serving the function as a
136 Milk Protein
natural scavenger and selective cytotoxic killer of cancerous cells in early infancy. The
development of normal epithelia cells in the digestive system could also be affected.
Obviously, many dairy products containsα-LA in fairly high concentrations and numerous
production techniques, such as acid pH and heat treatment, facilitate the formation of partly
unfoldedα-LA making the protein ready to bind fatty acids and other similar compounds.
To what degree such complexes in fact are formed in dairy products remains to be seen.
Its late, I am tired, I am so close to finding another set of instructions to make bamlet/hamlet.
If any of my wonderful readers finds this then, the prize is yours. Just add it as comment. Of course I am happy to recommend bravo probiotic and do, and will continue to do so, for those who can afford it. To be honest you basically buy shares in Prof Ruggiero's reputation when you buy the yogurt. He is trust worthy and very clever. But it's like everything in this life, it comes down to the MONEY. Thats the reason I am not doing therapies I should be here in Germany. So maybe together we will find the free version of HAMLET/BAMLET, I am pretty sure we can just add the colostrum and some saliva and we have activated GCMAF in a hamlet mix. Remember, if you are making the yogurt, you have to add the olive oil, it's not in Ruggieros earliest instructions either, so adding the Oleic acid is a recent improvement in the maf 314 world. Is it in the current Bravo material ?, I doubt it.
HAMLET and BAMLET. Maybe the maf 314 yogurt I have been making for over 18 months, is a key part of my survival, I think it might just be. All the people I given this to and they gave up, one day I will have proof of its utility beyond my own health with my curse. I note some friends started making the yogurt very late stage and with a serious infections, he has passed away now, so the yogurt does not perform late stage miracles. But for the group of cancer patients into functional foods, well I think this one fits the bill.
The human blader cancer invivo study. Now I think about trying to share maf 314 with the patients, staff at Hallwang clinic, 18 months earlier. That attempt failed completely. "I told you so" is so pathetic with all of my maf 314 failures. I am just reflecting on how set in our ways we become, I guess I am no different, I am grateful I was just lucky to get set in some effective healing ways early on. So despite considerable challenges, I am still optimistic I can smash this metastatic colorectal and get back to scuba diving real soon and entire journey will just be an interesting chance to dry out, so to speak. Of course I could be wrong and we know what that leads to.
The focus of this blog is to share precisely what I am doing, why and the results. One day soon I will be cancer free, I will look back at the blog and cringe and be proud at the same time. I am so proud of my friends Rona blog, she has such a professional approach and millions of readers, as opposed to my single reader. Hi Mum and thanks, without you the statistics would look terrible. My wonderful friend Rona http://bisforbananascisforcancer.wordpress.com/ has a gift for picking out what's really important in my journey and sharing it effectively and all her other cancer news. Thats what I really appreciate, she shares so much interesting cancer news. It's a bit like commensural life between microbes in our gut, they have unique and complementary functions, just like us humans.
I actually I think all the cancer news though can be of limited value in some respects, I like information that I can translate into lifestyle therapies. Having a clever friend sort through the news and send over updates is so helpful. Like having olive oil with your original GCMAF to maximise macrophage activation, of course its possible this is just a fantasy, then again, it sounds reasonable to me, and I will DO IT. That's what has been the secret of my success so far, that's what I am going to keep on doing. Lots of little, simple positive lifestyle enhancements.
Another challenge, my work permit application was declined, so I will be going home on the 11th regardless, as I cannot over stay the visa. I need to put in a better application.
These are many false leads in research and science, and the road takes many twists and turns. It'sdawned on me that I can never really thank the scientists and doctors involved in all the research papers I have reviewed and reply on in so many ways. Today I steal a few wonderful discussions, I seek to provide the detailed evidence for the maf 314 probiotic yogurts, patients need to be educated, and then they will make it.
Bioactive properties and clinical safety of a novel milk protein peptide another fascinating paper, yes this is what I do with my life, I research and share knowledge that I believe instrumental in survival. I think that beyond sound holistic health practices, my focus is always on targeted therapies. Like maf 314 or bravo probiotic. With my Buddhist approach to life, I detest the greed that denies this truly excellent medicine to the poor of the world, it's especially interesting to note that it was in poor communities the yogurt was discovered, so there you have a gift a discovery from the poorest and the most desperate communities afflicted with aids maf 314 instructions and history. What does science do ? It seeks to commercialise this potentially beneficial discovery. Is this unethical in some respects, yes because I know many patients that would give this yogurt a try if was 100 for 3 months not 550 euro. On the other hand I am grateful that incentive exists, otherwise I would not have benefited and I maybe be dead, and the many who have benefited from the yogurt would be a lot worse off more than likely.
It seems early capitalists in this area of GCMAF like David Noakes and Marco Ruggiero provide a necessary service to bring this science publicity and attention, and make it commercially available, to run what limited studies have been completed to date. That said someone like Bill Gates, could come along, discover the cultures and make them freely available to the world. Its a nice prayer, I will add it to the list, you see GCMAF and our immune system has that potential I suspect to cure so many illnesses I pray I will be alive to see GCMAF takeover the health world. I am sure a new army of capitalists will come along and ensure the entire world benefits from GCMAF, I might even be one of them. Greed some say is good, so is competition, how the future plays out will be exciting.
Milk represents a unique source of nutrients and biologically active components that act in synergy, as well as independently. Emerging evidence indicates that the protein component of milk represents a variety of biologically active proteins/peptides that function as anti-hypertensive agents, antimicrobial factors, food intake modifiers and immune regulatory factors [8,9]. Interestingly, many bioactive peptides are inactive within their parent milk proteins, and upon release during digestion or food processing, they may act as regulatory compounds with hormone-like activity [10,11]. Additionally, there are increasing studies showing that bioactive milk peptides can be absorbed intact from the intestinal lumen into the blood circulation - these may thus serve as novel functional food ingredients or pharmaceutical agents [12-15].
Using a proprietary enzyme digestion and buffer isolation method, we purified a group of peptides from the whey fraction of regular cow's milk and screened against a panel of kinases. In particular, this milk peptide mixture shows inhibitory effects against EGFR, VEGFR2, and IR. EGFR is often overexpressed in non-small cell lung cancer (NSCLC) and a variety of common solid tumors. EGFR signaling is generally associated with cancer invasion, metastasis, chemotherapy resistance, and poor prognosis [16,17]. It has also been reported that inhibition of EGFR may lead to apoptosis in certain cancer cell lines [18]. Gefitinib (Iressa®, AstraZeneca plc, London, UK) and Erlotinib (Tarceva®, Genentech, Inc., San Francisco, CA) are examples of anti-cancer drugs targeting EGFR-TK. Interestingly, MP mixture is also able to cause significant cell death in HT29 colon cancer cells, whereas neither commercially available hydrolyzed whey proteins nor whole milk proteins exhibited the same property. This may be because most commercial hydrolyzed whey proteins contained too small amount of bioactive peptides or the process of spray drying deactivated the activity.
This novel MP mixture also inhibits insulin receptor signaling. Interestingly, mutations in daf-2, a gene that encodes an insulin-like receptor in C. elegans worm, have been shown to double the lifespan of the worms [19]. The gene is known to regulate reproductive development, ageing, resistance to oxidative stress, thermotolerance, resistance to hypoxia and also resistance to bacterial pathogens [20]. Therefore, we next tested this unique MP mixture effect on the lifespan of C. elegans worms. Statistical analysis of the result suggested that N2 worms fed with agar containing 20 μg/ml concentration of milk peptides increased the median lifespan by 15.4% (p = 0.014). Based on these data, we hypothesize that this milk peptide mixture may be a novel supplement ingredient for anti-aging and cancer preventive regimen.
Immunology Lecture 14 Type II Hypersensitivity Reactions
You need to watch this on Mobeens website, not sure why its moved. Getting a handle on the real illnesses helps put all the theories into context. I actually have started making notes on my medical notepad.
http://usmlestrategy99.com/usmle-step-1/immunology/item/57-type-2-hypersensitivity-article
Strategies to maximise original GCMAF given IV
Having some holistic fun, going to splurge and do a final IPT, so i will have my Xeloda for breakfast. Again based on the chemo/immune paper, we do cytotoxic and then follow by immune stimulation.
Take a diet rich in FAT, and lots of olive oil and my vit D drops at breakfast and do the GCMAF one full vile, and I will time some tablespoons of olive oil one hour before the GCMAF.
I am stopping all offlabels and supplements for the weekend, just in case any interference with GCMAF. I will also be hoping to achieve my own GOLEIC version by having the oleic acid freely available in my plasma, base on Prof Ruggieros explanation the protien exists in plasma not in a pure form but with oleic acid and vit D3 attached at various spots. I suspect the product known as Goleic is just the original GCMAF with a some pure oleic acid added, and that this needs to be shaken to be mixed and activated. I wonder how these acids and protiens go when mixed in the blood. Its interesting to see one of the health benefits of the mediterean diet might be immune activation of GCMAF due to the olive oil.
So my super diet, will have a really good greek salad with lots of olive oil, the dessert will then be dark chocolate with lots of GCMAF yogurt.
Acute appearance of fatty acids in human plasma – a comparative study between polar-lipid rich oil
from the microalgae Nannochloropsis oculata and krill oil in healthy young males
Results
Fatty acids derived mainly from the breakfast appeared rapidly in plasma, peaking about 3 hours after consuming the breakfast, and in a pattern that reflected their content in the breakfast. There were time-dependent increases in the concentrations of both EPA and DHA with both algal oil (P < 0.001 for EPA; P = 0.027 for DHA) and krill oil (P < 0.001 for both EPA and DHA). The concentration of EPA was higher with algal oil than with krill oil at several time points. DHA concentration did not differ between oils at any time point. The maximum concentration of EPA was higher with algal oil (P = 0.010) and both the area under the concentration curve (AUC) and the incremental AUC for EPA were greater with algal oil (P = 0.020 and 0.006). There was no difference between oils in the AUC or the incremental AUC for DHA.
Molecular mechanisms of the cytotoxicity of human α-lactalbumin made lethal to tumor cells (HAMLET) and other protein-oleic acid complexes. From these results, we conclude that the protein portion of HAMLET, GAMLET, and the other HAMLET-like protein-oleic acid complexes is not the origin of their cytotoxicity to tumor cells and that the protein portion of these complexes plays a role in the delivery of cytotoxic oleic acid molecules into tumor cells across the cell membrane.
Oleic Acid May Be the Key Contributor in the BAMLET-Induced Erythrocyte Hemolysis and Tumoricidal Action BAMLET was prepared by the heat-treatment procedure described by Kamijima et al. [20]. Briefly, α-LA was dissolved in phosphate-buffered saline (PBS), pH 7.4 to a concentration of 700 µM. OA (120 molar equivalents) was added to the protein solution, mixed gently and the mixture was incubated at 50 °C for 10 min. The tubes were cooled to 25 °C and centrifuged. After removal of excess OA by aspiration the preparation was subjected to dialysis against PBS for 18 h.
The synergy of ketogenic diet and Hamlet - I know the maf 314 makes Hamlet proteins, so i am assuming the bacteria are responsible for the manufacturing the hamlet complexes.
"Furthermore, HAMLET sensitivity was modified by the glycolytic state of tumor cells. Glucose deprivation sensitized tumor cells to HAMLET-induced cell death "
Abstract
HAMLET is the first member of a new family of tumoricidal protein-lipid complexes that kill cancer cells broadly, while sparing healthy, differentiated cells. Many and diverse tumor cell types are sensitive to the lethal effect, suggesting that HAMLET identifies and activates conserved death pathways in cancer cells. Here, we investigated the molecular basis for the difference in sensitivity between cancer cells and healthy cells. Using a combination of small-hairpin RNA (shRNA) inhibition, proteomic and metabolomic technology, we identified the c-Myc oncogene as one essential determinant of HAMLET sensitivity. Increased c-Myc expression levels promoted sensitivity to HAMLET and shRNA knockdown of c-Myc suppressed the lethal response, suggesting that oncogenic transformation with c-Myc creates a HAMLET-sensitive phenotype. Furthermore, HAMLET sensitivity was modified by the glycolytic state of tumor cells. Glucose deprivation sensitized tumor cells to HAMLET-induced cell death and in the shRNA screen, hexokinase 1 (HK1), 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 and hypoxia-inducible factor 1α modified HAMLET sensitivity. HK1 was shown to bind HAMLET in a protein array containing ∼8000 targets, and HK activity decreased within 15 min of HAMLET treatment, before morphological signs of tumor cell death. In parallel, HAMLET triggered rapid metabolic paralysis in carcinoma cells. Tumor cells were also shown to contain large amounts of oleic acid and its derivatives already after 15 min. The results identify HAMLET as a novel anti-cancer agent that kills tumor cells by exploiting unifying features of cancer cells such as oncogene addiction or the Warburg effect.
- PMID: